Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (2): 260.

• Articles • Previous Articles     Next Articles

Construction and Application of CdTe Quantum Dots-Rhodamine 6G Fluorescence Resonance Energy Transfer Systems

WANG Xu-Yan, LIANG Jian-Gong, MA Jin-Jie, CHEN Shu-Han, HAN He-You*   

  1. College of Science, State Key Laboratroy of Agricatrual Microbiology, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2009-09-27 Online:2010-02-10 Published:2010-02-10
  • Contact: HAN He-You. E-mail: hyhan@mail.hzau.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20975042)、转基因科技重大专项基金(批准号: 2009ZX08012-015B)、湖北省自然科学基金重点项目(批准号: 2008CDA080)及湖北省自然科学基金面上项目(批准号: 2008CDB031)资助.

Abstract:

Two kinds of thiols modified CdTe quantum dots were used to construct the CdTe quantum dots(donor)-Rhodamine 6G(acceptor) fluorescence resonance energy transfer(FRET) systems, which were applied to investigate the interaction mechanism between CdTe quantum dots and bovine serum albumin(BSA). The results showed that the energy transfer efficiency of the CdTe quantum dots-rhodamine 6G FRET systems were improved, and the distance between CdTe quantum dots and rhodamine 6G(r) were decreased after CdTe quantum dots interacted with BSA, BSA were directly binding to the surface of quantum dot by the coordination between its tryptophan(Trp) residues and the metal.

Key words: CdTe quantum dot; Rhodamine 6G; Fluorescence resonance energy transfer; Bovine serum albumin

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