Chem. J. Chinese Universities ›› 2010, Vol. 31 ›› Issue (2): 231.

• Articles • Previous Articles     Next Articles

Interaction of both Indole and Imidazole Group-containing Ruthenium(Ⅱ) Complex with Yeast-RNA

YANG Huai-Xia1,2, LIU Yan-Ju1,2, ZHAO Lin1, WANG Ke-Zhi2*   

  1. 1. Pharmacy College, Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China;
    2. College of Chemistry, Beijing Normal University, Beijing 100875, China
  • Received:2009-04-27 Online:2010-02-10 Published:2010-02-10
  • Contact: WANG Ke-Zhi. E-mail: kzwang@bnu.edu.cn
  • Supported by:

    国家自然科学基金(批准号: 20771016, 20971016, 90922004)、北京市自然科学基金(批准号: 2072011)、高等学校博士学科点专项科研基金(批准号: 20060027002)和北京师范大学分析测试基金资助.

Abstract:

The binding of a novel ruthenium(Ⅱ) complex of [Ru(bpy)(H2iip)2](ClO4)2{bpy=2,2′-bipyridyl, H2iip=2-(1H-indole-3-yl)-1H-imidazo[4,5-f][1,10]-phenanthroline}, to yeast-RNA was investigated by UV-Visible and emission spectroscopy titrations, steady-state emission quenching and competitive binding with ethidium bromide experiments. The results suggest that the binding of this complex to the RNA be intercalative with a binding constant of 7.09×106 L/mol, which is higher than that to calf thymus DNA and the RNA binding constant of an analogous complex [Ru(bpy)2(H2iip)](ClO4)2.

Key words: Ruthenium(Ⅱ); Complex; Yeast-RNA; Indole; Bipyridine

TrendMD: