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Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (9): 1739.

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NMR Analysis of Polysaccharide ASP3 from Angelica sinensis(Oliv.) Diels and It′s Hydrolysis Products

SUN Yuan-Lin1*, SHAN Fang2, CUI Wu-Wei3, TANG Jian4, GU Xiao-Hong4   

  1. 1. Department of Life Science, Yuncheng University, Yuncheng 044000, China;
    2. Institute of Farm Products Comprehensive Utilization, Shanxi Academy of Agricultural Sciences, Taiyuan 030031, China;
    3. Food Research Program, Agriculture and Agri-Food, Ontario N1G 5C9, Canada;
    4. Key Laboratory of Food Science and Safety, Ministry of Education, Jiangnan University , Wuxi 214036, China
  • Received:2009-03-04 Online:2009-09-10 Published:2009-09-10
  • Contact: SUN Yuan-Lin. E-mail: sylwts@yahoo.com.cn
  • Supported by:

    山西省青年科研基金(批准号: 2007021042)、院级重点科研基金(批准号: 20060102)和院级学术带头人基金资助.

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Abstract:

The structural features of polysaccharide ASP3 were studied. ASP3 was hydrolyzed with 0.2 mol/L trifluoroacetic acid and was enzymic hydrolyzed with endo-α-(1→4)-polygalacturonases. NMR analysis method was conducted on the samples. The results indicated that ASP3 was a kind of pectic polysaccharide which contained the main part of homogalacturonan fragments as “smooth regions” with α-(1→4)-GalpA, and rhamnogalacturonan segments as “hairy regions” backbone. Gal and Ara, which were the mainly constitution of the branch, substituted at O4 position of Rhap residues in the backbone. Man and Glc were linked as arabinogalactan, galactan and arabinan. Terminal, β-(1→3)-, β-(1→3,6)-, β-(1→4)-, and β-(1→4,6)-Galp as well as residues of terminal, α-(1→5)-, and α-(1→3,5)-Araf were linked to form β-(1→6)- or β-(1→4)-galactans bearing 3,6- or 4,6-substituted β-Galp residues as branched points respectively, and short α-(1→5)-arabinan possessing 3,5-substituted α-Ara residues as branched points. In addition, arabinogalactan was found to contain branched sugar chains of α-(1→5)-arabinan attached to the linear chains of β-(1→6)-galactan by 1,3-linkages.

Key words: Angelica sinensis; Pectic polysaccharide; Partial acid hydrolysis; Enzymic digestion; NMR

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