CJCU

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (8): 1577.

• Articles • Previous Articles     Next Articles

Chiral Resolution of D,L-Valine by Immobilized Cells with Acetylornithine Deacetylase Activity

ZHANG Fei1,2, XIONG Ji-Bing1, LIU Jun-Zhong1, LIU Peng-Gang1, JIAO Qing-Cai1*   

  1. 1. State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China;
    2. Baotou Light Industry Vocational Technical College, Baotou 014043, China
  • Received:2008-12-30 Online:2009-08-10 Published:2009-08-10
  • Contact: JIAO Qing-Cai. E-mail: jiaoqc@yahoo.com.cn
  • Supported by:

    国家技术创新基金(批准号: 02CJ-13-01-16) 资助.

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Abstract:

D-Amino acids are widely used intermediates in pharmacy. D-Amino acids can be produced by chiral separation of D,L-amino acids racemate. The stereospecific hydrolysis of N-acetyl-D,L-amino acids by microbial enzymys is one of the most extensively used procedures in optical resolution of D,L-amino acids. In this report, a new method of chiral resolution of D,L-valine by immobilized cells with acetylornithine deacetylase activity was developed. The optimum reaction conditions were 200 mmol/L N-acetyl-D,L-valine and 0.2 g/mL(or 100 U/mL) of immobilized cells with 0.1 mmol/L CoCl2 as activator at pH=6. After 2—3 h incubation at 50 ℃, 95 mmol/L L-valine was obtained. In continuous use of immobilized cells for 10 times, the average conversion rate against N-acetyl-L-valine was 90.8%. The results display great potential in industrialization. This is the first report of chiral resolution of D,L-valine by acetylornithine deacetylase(ArgE).

Key words: Acetylornithine deacetylase; Chiral resolution; Immobilized cell; D,L-Aline

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