Abstract:

A novel fluorescence-based method for protein detection was developed based on aptamer probe as the recognized molecule and cationic conjugated polymers(CCP) as the reporter. The quencher-labeled aptamer probe attached to CCP by electrostatic interaction, leading to fluorescence quenching of CCP. When target protein was added, it bonded specifically to aptamer probe, which made the quencher detached from CCP. Then the fluorescence of CCP was resumed. The results show that the fluorescence resuming ratio is proportional to the concentration of target protein. The detection method for thrombin had a linear range of 17—40 nmol/L.

Key words: Aptamer; Thrombin; Cationic conjugated polymers; Fluorescence detection