Isolation and Structure Characterization of Polysaccharide from Morinda officinalis How

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (12): 2391.

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Isolation and Structure Characterization of Polysaccharide from Morinda officinalis How

HE Chuan-Bo^1*, LI Lin², TANG Feng-Xia¹, XIONG He-Jian¹

1. School of Bioengineering, Jimei University, Xiamen 361021, China;
2. School of Light Chemistry and Food Science, South China University of Technology, Guangzhou 510640, China

Received:2009-04-09 Online:2009-12-10 Published:2009-12-10
Contact: HE Chuan-Bo. E-mail: hcbcc@sina.com
Supported by:
国家自然科学基金(批准号: 20436020)和广东省科技攻关项目(批准号: 2003C104025)资助.

Abstract

Abstract:

Morinda officinalis How is a rare Chinese traditional herbal medicine. A water soluble polysaccharide(MOPI-3) was obtained from Morinda officinalis How, by hot water extraction, deproteination with Sevag method, precipitation with ethanol, and fractionation through DEAE-Sepharose CL-6B ion exchange chromatography. Using UV, IR, NMR, GC-MS, periodate oxidation, Smith degradation and methylation reaction, the primary structure of MOPI-3 was identified. The results show that, MOPI-3 is highly purified with average molecular weight of 35309 determined by high performance gel permeation chromatography. And it is a heteropolysaccharide composed of Ara, Gal and Glc. The backbone of MOPI-3 was linked by α-1,3-pyran-glucose and α-1,4-pyran-galactose, and the molar ratio of glucose to galactose was 5:1. There was one side chain composed by three α-1,3-furan-arabinoses linked onto C6 of glucose on backbone in each repeat unit, and the O-acetyl group was linked onto C2 of galactose.

Key words: Morinda officinalis How; Polysaccharide; Isolation; Structure characterization

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HE Chuan-Bo*, LI Lin, TANG Feng-Xia, XIONG He-Jian. Isolation and Structure Characterization of Polysaccharide from Morinda officinalis How[J]. Chem. J. Chinese Universities, 2009, 30(12): 2391.

References

[1]YAO Hui(姚辉), ZHAO Sheng(赵晟). Plants(植物杂志)[J], 2002, (1): 26—27
[2]WU Yan-Bin(吴岩斌), WU Jin-Zhong(吴锦忠), ZHANG Qiao-Yan(张巧艳), et al.. Pharm. J. Chin. PLA(解放军药学学报)[J], 2009, 25(1): 64—67
[3]YANG Li-Hui(杨黎辉), YAN Fu-Man(闫福曼), CHEN Chao-Feng(陈朝凤), et al.. Acta Chinese Medicine and Pharmacology(中医药学报)[J], 2005, 33(2): 6—8
[4]WANG Yan-Fang(王燕芳), WU Zhao-Hua(吴照华), ZHOU Xin-Yue(周新月), et al.. Acta Botanica Sinica(植物学报)[J], 1986, 28(5): 566—569
[5]ZHAO Yi(赵诣), GUO Su-Hua(郭素华). Strait Pharmaceutical Journal(海峡药学)[J], 2007, 19(2): 59—60
[6]WANG Wei-Ping(王卫平). Lishizhen Medicine and Materia Medica Research(时珍国医国药)[J], 2000, 11(7): 665—666
[7]CUI Cheng-Bin(崔承彬), YANG Ming(杨明), YAO Zhi-Wei(姚志伟), et al.. Chinese Journal of Medicinal Chemistry(中国药物化学杂志)[J], 1995, 5(1): 32—39
[8]Zhong Q. Z., Li Y., Ming Y., et al.. Pharmacology, Biochemistry and Behavior[J], 2002, 72: 39—43
[9]Yun F. L., Zheng H. G., Ming Y., et al.. Life Sciences[J], 2003, 72: 933—942
[10]GU Xiao-Mei(顾笑梅), WU Hou-Ming(吴厚铭), MA Gui-Rong(马桂荣). Chem. J. Chinese Universities(高等学校化学学报)[J], 2004, 25(7): 1288—1290
[11]ZHANG Wei-Jie(张惟杰). Biochemistry Research and Technology of Glycoconjugates, 2nd Edition(糖复合物生化研究技术, 第2版)[M], Hangzhou: Zhejiang University Press, 2003: 37—38; 193—198
[12]Hakomori S. J.. Biochem.[J], 1964, 55: 205—208
[13]Fundin J., Weintraub A., Xu Jian-Guo, et al.. Magnetic Resonance in Chemistry[J], 2003, 41: 202—205
[14]Faber E. F., van den Haak M. J., Kamerling J. P., et al.. Carbohydrate Research[J], 2001, 331: 173—182
[15]SHI Lei(石磊), CHEN Kao-Shan(陈靠山), DONG Qun(董群), et al.. Chem. J. Chinese Universities(高等学校化学学报)[J], 2007, 28(6): 1088—1091
[16]Stefan W., Bjarne H.. Wood Sci. Technol.[J], 2004, 38: 173—179

Isolation and Structure Characterization of Polysaccharide from Morinda officinalis How

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