CJCU

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (12): 2371.

• Articles • Previous Articles     Next Articles

Leukemic Cell-enzyme-linked Immunosorbent Assay Using Interdigitated Electrodes Array with Enzymatic Silver Deposition

TAN San-Qin1,2, WANG Guang-Ping1, JIANG Jian-Hui1, SHEN Guo-Li3, ZHANG Rong4, PENG Ming-Yuan1, JIN Xiao-Yong3, FU Gan1, CHEN Fang-Ping1*   

  1. 1. Department of Hematology of Xiangya Hospital, Central South University, Changsha 410082, China;
    2. Department of Histology and Embryology of Medical College, Hunan Normal University, Changsha 410006, China;
    3. State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering,
    4. Research Center of Micro-Nano Technology, Hunan University, Changsha 410082, China
  • Received:2009-07-15 Online:2009-12-10 Published:2009-12-10
  • Contact: CHEN Fang-Ping. E-mail: xychenfp@2118.cn
  • Supported by:

    湖南省科技支撑计划重点项目(批准号: 07SK2002)资助.

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Abstract:

An cell-based ELISA for cell surface antigen using interdigitated electrode array with enzymatic silver deposition was developed. Cell suspension were added to each well of a 96-well clear plate(poly-L-lysine coated).The cells were incubated with mouse-anti-human IgG, and then with HRP-conjugated horse-anti- mouse IgG. The interdigitated electrode array was added, the deposition of silver was dispersed over the microgaps and allows the microgapped interdigitated electrodes to be electrically connected, resulting in an increase in electrical conductance of interdigitated electrode array that is used to quantify the cell surface antigen.The electrical conductance signal showed correlation with cell surface antigen.Our results revealed that the electrochemical immunoassay, which was enabling the large-scale analysis of cell surface antigen, has characteristics(analyte specific, low background and low limit of detection) that provide potential for typing of acute leukemia(AL). This novel electrochemical immunoassay can also be applied in measuring the binding activity of engineering antibodies of cell surface molecules.

Key words: Electrochemical immunoassay; Enzymatic silver deposition; Interdigitated electrodes array; Cell surface antigen

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