Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (11): 2189.

• Articles • Previous Articles     Next Articles

Isolation, Purification and Structural Analysis of Polymannuronic Acid from Undaria pinnatifida

WANG Cai1, SHI Song-Shan1, HONG Qi-Ming2, WANG Shun-Chun1*, WANG Zheng-Tao1, HU Zhi-Bi1   

  1. 1. Key Laboratory of Standardization of Chinese Medicines, Ministry of Education, Institute of Chinese Materia Medica,
    2. College of Pharmacy, Shanghai University of Traditional Chinese Medicine, Shanghai 201210, China
  • Received:2009-03-19 Online:2009-11-10 Published:2009-11-10
  • Contact: WANG Shun-Chun. E-mail: shunchunwang@126.com
  • Supported by:

    国家“八六三”计划(批准号: 2006AA090405)和上海市曙光计划项目(批准号: 08SG44)资助.

Abstract:

UPPS03 and UPPS04 were obtained from Undaria pinnatifida by water extraction, DEAE-Sepharose Fast Flow, Sephacryl S-300 and Sephacryl S-200 gel permeation chromatography. HPGPC shows that both of them are homogeneous polysaccharides with an average molecular weigh of 3.6×104 and 1.1×104, respectively. The structural features of UPPS03 and UPPS04 were characterized with sugar composition analysis, periodic acid oxidation and Smith degradation, reduction of uronic acid, methylation analysis, IR and NMR. The results indicate that they are all linear polysaccharide with β-1,4-linked polymannuronic acid, and this is the first report of polymannuronic acid isolated from Undaria pinnatifida.

Key words: Undaria pinnatifida; Polymannuronic acid; Isolation and purification; Structural analysis

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