Sandwich Immunosensing Method for Measuring Cardiac Troponin I with Quantum Dots-coupled Antibody Against Troponin I

Chem. J. Chinese Universities ›› 2009, Vol. 30 ›› Issue (10): 1940.

• Articles • Previous Articles Next Articles

Sandwich Immunosensing Method for Measuring Cardiac Troponin I with Quantum Dots-coupled Antibody Against Troponin I

SONG Jian^1,2, FAN Jia², SONG Da-Qian³, BI Li-Rong⁴, ZHOU Gang-Yu⁴, ZHANG Hao³, WEI Jing-Yan^2,3*, YANG Bai³

1. College of Electronic Science and Engineering, Jilin University, Changchun 130012, China;
2. College of Pharmacy, Jilin University, Changchun 130021, China;
3. College of Chemistry, Jilin University, Changchun 130012, China;
4. China-Japan Union Hospital of Jilin University, Changchun 130033, China

Received:2009-06-24 Online:2009-10-10 Published:2009-10-10
Contact: WEI Jing-Yan. E-mail: weijy@jlu.edu.cn
Supported by:
国家自然科学基金(批准号: 29875010)和吉林省科技厅科研基金(批准号: 200705380)资助.

Abstract

Abstract:

In this study, a novel quantitative sandwich immunoassay for detecting cTn I was developed by using surface plasmon resonance biosensor and quantum dots(QD)-coupled antibody for the first time, which amplified the response of biosensor and so had the sensitivity of this method obviously improved. First quantum dots were coupled to the monoclonal antibody(2F11) against cTn I with N-hydroxysuccinimide(NHS) and 1-ethyl-3-(3-dimethyl-aminopropyl)-carbodimidehydrochloride(EDC), and then the coupled product(QD-2F11) was confirmed by SDS-PAGE and its antibody activity was tested by protein blot. Finally, a sensitive sandwich immunosensing method for measuring cardiac troponin I was developed by surface plasmon resonance biosensor with protein A as an intermediate layer, the specific polyclonal antibody as the first antibody[capture antibody, and QD-labeled-monoclonal antibody as the second antibody(detecting antibody)]. The novel sandwich immunoassay showed the detection range of 0.4 to 15 μg/L and a detection limit of0.4 μg/L, which is higher 2 times than the sandwich immunoassay without using QD-labeled antibody and 10 times than the direct immunoassay for cTn I previously developed, respectively.

Key words: Cardiac troponin I; Specific antibody against cardiac troponin I; Sandwich immunosensing me-thod; Quantum dot; Biosensor

TrendMD:

SONG Jian, FAN Jia, SONG Da-Qian, BI Li-Rong, ZHOU Gang-Yu, ZHANG Hao, .... Sandwich Immunosensing Method for Measuring Cardiac Troponin I with Quantum Dots-coupled Antibody Against Troponin I[J]. Chem. J. Chinese Universities, 2009, 30(10): 1940.

References

[1]Wu X., Liu H., Liu J., et al.. Nature Biotechnology
[J], 2002, 21: 41—46

[2]Bruchez M. J., Moronne M., Gin P., et al.. Science
[J], 1998, 281(5385): 2013—2016

[3]Chan W., Nie S. M.. Science
[J], 1998, 281(5385): 2016—2018

[4]Seydel C.. Science
[J], 2003, 300(5616): 80—81

[5]Wilkinson J. M., Grand R. J. A.. Nature
[J], 1978, 271: 31—35

[6]Vallins W. J., Brand N. J., Dabhade N., et al.. FEBS Lett.
[J], 1990, 270: 57—61

[7]Zaninotto M., Altinier S., Lachin M., et al.. Clin. Chem.
[J], 1996, 42: 1460—1466

[8]WEI Jing-Yan(魏景艳), MU Ying(牟颖), XU Hai-Bo(许海波), et al.. Chem. J. Chinese Universities(高等学校化学学报)
[J], 2003, 24(12): 2161—2164

[9]JIANG Jian-Ping(姜建平), WANG Zi-Yu(王子瑜), DENG Hai-Feng(邓海峰), et al.. Shanghai J. Immunol.(上海免疫学杂志)
[J], 1999, 19: 49—50

[10]WEI Jing-Yan(魏景艳), SONG Da-Qian(宋大千), BU Li-Sha(卜丽莎), et al.. Chin. J. Immunol.(中国免疫学杂志)
[J], 2004, 20(4): 272—275

[11]TIAN Yuan(田媛), CHEN Yan-Hua(陈艳华), BI Shu-Yun(毕淑云), et al.. Chin. J. Anal. Chem.(分析化学)
[J], 2006, 34(7): 967—970

[12]TAO Lei(陶磊), JIN Wei(金伟), ZHANG Ying(张莹), et al.. Chem. J. Chinese Universities(高等学校化学学报)
[J], 2009, 30(5): 882—885

[13]Mu Y., Zhang H. Q., Zhao X. J., et al.. Sensors
[J], 2001, 1: 91—101

[14]ZHAO Xiao-Jun(赵晓君), MU Ying(牟颖), WANG Zhen(王珍), et al.. Chem. J. Chinese Universities(高等学校化学学报)
[J], 1999, 20(4): 704—708

[15]SONG Da-Qian(宋大千), MU Ying(牟颖), ZHAO Xiao-Jun(赵晓君), et al. Chem. J. Chinese Universities(高等学校化学学报)
[J], 2000, 21(4): 686—689

[16]Larue C., Defacque-Lacquement H., Calzolari C., et al.. Molecular Immun.
[J], 1992, 29: 271—278

[17]Bodor G. S., Porter S., Landt Y., et al.. Clin. Chem.
[J], 1992, 38(11): 2203—2214

[18]WEI Jing-Yan(魏景艳), FU Ping-Ping(付平平), SONG Ang(宋昂). Chin. J. Biologicals(中国生物制品学杂志)
[J], 2000, 13(4): 218—220

[19]WEI Jing-Yan(魏景艳), QIAN Li-Na(钱丽娜), LU Jie(芦杰), et al.. Chin. J. Biologicals(中国生物制品学杂志)
[J], 2002, 15(5): 29—31

[20]WEI Jing-yan, SONG Da-qian, BU Li-sha, et al.. Chem. Res. Chinese Universitis
[J], 2003, 19(2): 183—189

[21]Hua X. F., Liu T. C., Cao Y. C., et al.. Analytical and Bioanalytical Chemistry
[J], 2006, 386(6): 1665—1671

[22]Besselink G. A., Kooyman R. P., van Os P. J., et al.. Anal. Biochem.
[J], 2004, 333: 165—173

Sandwich Immunosensing Method for Measuring Cardiac Troponin I with Quantum Dots-coupled Antibody Against Troponin I

PDF (PC)

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 0

Recommended Articles

Metrics

Comments