Preparation and Evaluation of the Biotinylated Polymer Coating

Chem. J. Chinese Universities

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Preparation and Evaluation of the Biotinylated Polymer Coating

LU Tian-Cheng^1,2, DENG Chao^1,2, SUN Jing^1,2, CHEN Xue-Si¹, ZHANG Pei-Biao¹, JING Xia-Bin¹*

1. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China

Received:2007-11-08 Revised:1900-01-01 Online:2008-04-10 Published:2008-04-10
Contact: JING Xia-Bin

Abstract

Abstract: A novel bioactive polymer coating without nonspecific adsorption of proteins was prepared from a biodegradable amphiphilic triblock copolymer, biotinylated poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-lysine)(PEG-PLA-PLL/biotin) by coating it on a substrate and covering it with a layer of blocking coating consisting of gelatin and poly(N-vinyl pyrrolidone). The bioactive surface was characterized by enzyme linked immunoassay, on the basis of sequential immobilizations of horseradish peroxidase labeled streptavidin, biotinylated mouse globulin, and alkaline phosphatase labeled horse anti mouse IgG(H+L). The results showed that the bioactive surface was resistant to nonspecific protein adsorption but allowed specific recognition and combination between biotin and streptavidin, and meanwhile, the combined streptavidin retained its bioactivity. Therefore, subsequent protein immobilization can be realized via the reaction of the streptavidin and biotinylated proteins.

Key words: Bioactive coating, Biotin, Streptavidin, Protein adsorption, Protein immobilization, Immunoassays

CLC Number:

O633.14

TrendMD:

LU Tian-Cheng^1,2, DENG Chao^1,2, SUN Jing^1,2, CHEN Xue-Si¹, ZHANG Pei-Biao¹, JING Xia-Bin¹*. Preparation and Evaluation of the Biotinylated Polymer Coating[J]. Chem. J. Chinese Universities, doi: .

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Preparation and Evaluation of the Biotinylated Polymer Coating

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