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Relationship Between Solvated Polymer Peak and Solvent Peak Using Mixed Solvent as Eluents in Size-exclusion Chromatography

WANG Qing-Guo1*, LI Xiao-Wen1, LIU Bo1, CAI Li-Xing1 , CHENG Rong-Shi2,3   

    1. College of Chemistry and Technology, Shenzhen University, Shenzhen 518060, China;
    2. College of Chemistry and Technology, Nanjing University, Nanjing 210008, China;
    3. Research Institute of Polymer Science, South China University of Technology, Guangzhou 510640, China
  • Received:2007-04-28 Revised:1900-01-01 Online:2008-03-10 Published:2008-03-10
  • Contact: WANG Qing-Guo

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Abstract: In Size-exclusion Chromatography(SEC) two peaks are often observed for polymer samples when solvent mixtures are employed as eluents. These two peaks are solvated polymer peak and free solvent peak respectively. Theoretical analysis shows that the peak area of the solvated polymer A3eff is actually the sum of the “naked polymer” peak area A3 and the bound solvent peak area A1*. A1* is equal to that of the free solvent peak A2* in magnitude but opposite in sign. In this study the data of A3eff, A3 and A2* were measured for the system of polystyrene(3)-chloroform(1)-methanol(2). The results support the analytical prediction and indicate that the preferential adsorption coefficient can be determined from either the polymer peak or the free solvent peak.

Key words: Size-exclusion chromatography, Preferential solvation, Solvent mixture

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