Abstract: To explore the protein-level action mechanism of verapamil in acute myocardial infracted rats, the myocardial proteome was analyzed by two-dimensional electrophoresis(2-DE). In comparison with the sham-operated group and the infracted group, the result shows that eight proteins expressions in verapamil treated group were up-regulated, and seven proteins expression in this group were down-regulated significantly. By using MALDI-TOF-MS, 15 proteins with significant changes were identified by database searching. These proteins can be divided into four groups via their biological function: (1) energy metabolism and mitochondrial function related proteins; (2) oxidative stress-induced proteins; (3) cytoskeletal proteins; (4) other proteins. The findings show that the myocardial protective effects of verapamil in myocardial damage process were related to the recovery of energy supply as well as anti-oxidative stress property.

Key words: Verapamil, Proteomics, Two-dimensional electrophoresis(2-DE)