Application of HPLC-ICP-MS in Speciation Analysis of Selenium in Selenized Flammulina velutipes

Chem. J. Chinese Universities

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Application of HPLC-ICP-MS in Speciation Analysis of Selenium in Selenized Flammulina velutipes

TIE Mei¹, FANG Yu-Zhi², SUN Tie-Biao¹, LI Chuang¹, FEI Jin-Yan¹, LI Hua-Wei³, ZANG Shu-Liang^1*

1. Department of Environmental Science, Liaoning University, Shenyang 110036, China;
2. Department of Chemistry, East China Normal University, Shanghai 200062, China;
3. College of Environmental and Life Sciences, Shenyang Normal University, Shenyang 110034, China

Received:2006-08-06 Revised:1900-01-01 Online:2007-04-10 Published:2007-04-10
Contact: ZANG Shu-Liang

Abstract

Abstract: The determination of selenium in different selenium-containing compounds extracted from selenized Flammulina velutipes was studied by SEC-DADICP-MS-ICP-MS. After hydrolyzation of the different compounds under special condition, selenium-amino acid in the leaching solution was confirmed by RPLC-DADICP-MS-ICP-MS. A new selenium-containing compound was detected and the content of selenium in differnt offspring by hydrolyzation was determined. It is shown that the soluble selenium was the main existing form in selenized Flammulina velutipes, and the content of selenium in compounds low molecular weight accounting for 71.87% of the total amount was much higher than that in compounds with a high molecular weight. Then the further results show that in selenized Flammulina velutipes there mainly are Se-Cys, Se-Met, peptide composed by the former two. The max content of selenium in the three main speciations accounted for 12.3%, 17.6%, 36.8%. This method combined by HPLC and better sensitive element detect technology can be successfully applied in the online determination of selenium in selenium-containing biological molecule.

Key words: Flammulina velutipes, Speciation analysis, Selenium, HPLC-ICP-MS

CLC Number:

O657.63

TrendMD:

TIE Mei¹, FANG Yu-Zhi², SUN Tie-Biao¹, LI Chuang¹, FEI Jin-Yan¹, LI Hua-Wei³, ZANG Shu-Liang^1*. Application of HPLC-ICP-MS in Speciation Analysis of Selenium in Selenized Flammulina velutipes[J]. Chem. J. Chinese Universities, doi: .

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Application of HPLC-ICP-MS in Speciation Analysis of Selenium in Selenized Flammulina velutipes

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