Mini Microwave Resonance Cavity Used for Microwave-accelerated Protein-enzymolysis in Protein Mapping by Mass Spectrometry

Chem. J. Chinese Universities

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Mini Microwave Resonance Cavity Used for Microwave-accelerated Protein-enzymolysis in Protein Mapping by Mass Spectrometry

DOU Wen-Chao, HUAN Yan-Fu, ZHANG Zhi-Quan, ZHANG Hua-Rong, WANG Chao, LI Ming, FENG Guo-Dong, JIN Qin-Han

Jilin Province Research Center for Engineering and Technology of Spectral Instruments, College of Chemistry, Jilin University, Changchun 130021, China

Received:2006-06-21 Revised:1900-01-01 Online:2007-02-10 Published:2007-02-10
Contact: FENG Guo-Dong

Abstract

Abstract: A mini microwave resonance cavity was used to accelerate proteolytic cleavage of cytochrome c and bovine serum albumin. The resulting mixture were then analyzed by electrospray ionization mass spectrometer(ESI-MS).The digestion efficiencies of proteins was 100%,the sequence coverages of cytochrome c and bovine serum albumin were 45% and 26%, respectively. This approach not only speeds up the digestion process to 20 minutes versus 16 h by using traditional methods, but also reduced the power of microwave to 20 W versus hundreds Watt by using other microwave-assisted enzymatic digest methods.

Key words: Microwave resonance cavity, Microwave-accelerated protein digestion, Mass spectrometry, Selective endoprotease reactions

CLC Number:

O652.7

TrendMD:

DOU Wen-Chao, HUAN Yan-Fu, ZHANG Zhi-Quan, ZHANG Hua-Rong, WANG Chao, LI Ming, FENG Guo-Dong, JIN Qin-Han. Mini Microwave Resonance Cavity Used for Microwave-accelerated Protein-enzymolysis in Protein Mapping by Mass Spectrometry[J]. Chem. J. Chinese Universities, doi: .

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Mini Microwave Resonance Cavity Used for Microwave-accelerated Protein-enzymolysis in Protein Mapping by Mass Spectrometry

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