Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (5): 826.doi:
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ZHOU Cui-Song 1, 2, JIANG Ya-Xin2, WANG Jun2, MA Bao-Cheng2, LI Meng-Long1*, FANG Xiao-Hong 2*
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Abstract:
Aptamers are a new class of oligonucleotides generated from in vitro selection with a high affinity and specificity. In this paper, a novel luminescence detection method was developed by using RNA aptamers and a DNA light switching complex, [Ru(phen)2(dppz)]2+, as a signaling aptamer for the detection of drug tobramycin. The results showed that the method was highly sensitive and selective with the detection limit down to 10 nmol/L. The method was also very simple and could realize direct detection without separation in solution, and offers a new approach for the exploitation and application of aptamers in drug analysis.
Key words: Aptamer; Tobramycin; Luminescence spectroscopy; Light-switching complex; RNA
CLC Number:
O657.31
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ZHOU Cui-Song 1, 2, JIANG Ya-Xin2, WANG Jun2, MA Bao-Cheng2, LI Meng-Long1*, FANG Xiao-Hong 2*. High Sensitive Tobramycin Detection Using a Novel Signaling Aptamer[J]. Chem. J. Chinese Universities, 2006, 27(5): 826.
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http://www.cjcu.jlu.edu.cn/EN/Y2006/V27/I5/826
