CJCU

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (4): 635.

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Comparative  Analysis of Proteomes for Chemoresistance in Human Ovarian Adenocarcinoma Cell Line,  COC1/DDP

LU Lei 1,2,3, LIU  Zhi-Qiang 1* , LI Li 1 , LIU Ning1, LIU Shu-Ying1   

  1. 1. Laboratory of  New Drug Research and Mass Spectrometry, Changchun Institute of Applied  Chemistry, Changchun Center of Mass Spectrometry, Changchun 130022,  China; 2. Department of Chemistry,
    Yan′an University, Yan′an 716000, China; 3. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
  • Received:2005-11-24 Online:2006-04-10 Published:2006-04-10
  • Contact: LIU Zhi-Qiang,E-mail: liuzq@ciac.jl.cn

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Abstract:

The proteomic maps of  human ovarian adenocarcinoma cell line  in Human COC1 and its cell subline COC1/DDP were produced by 2-DE SDS-PAGE. The image analysis revealed obviously differential protein expression of the cells in response to cisplatin treatment. Approximate 1300 protein spots were resolved in the two ranges of pI(3—10). The spots which have 10-fold difference in light density were digested by in-gel enzyme, identified by matrix assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS) and finally detected by Profound and Mascot search engine in IDI databases. Seven proteins with obviously altered abundances were detected specifically, including four up-regulated protein spots and three down-regulated protein spots. The proteins differentially expressed in the parent cell line COC1 and its subline COC1/DDP were considered to be important for the  therapy and pathogenesis.

Key words: Ovarian adenocarcinoma; Proteome; Mass spectrum

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