CJCU

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (2): 268.

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Investigation of the Effect of 5-FU on p33ING1 mRNA Level Based on Molecular Beacon

WANG Wei,  LIU Bin,  LI Jun,  WANG Ke-Min*,  TAN Wei-Hong,  TANG Hong-Xing   

  1. State Key Laboratory of Chemo/Biosensing and Chemometrics,  Hunan Engineering Research Center for
    Bio-Nanotechnology,  Biomedical Engineering Center, Hunan University,  Changsha  410082, China
  • Received:2005-06-22 Online:2006-02-10 Published:2006-02-10
  • Contact: WANG Ke-Min，E-mail: kmwang@hnu.cn

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Abstract:

Abstract In order to conduct the fundamental investigation of the effect of 5-FU on HNE1 cells,  p33ING1 mRNA level in cellular total RNA was detected quantitatively based on MB assay.  During the operation,  the effects of 5-FU concentrations and treatment time in HNE1 cells and HNE1 cells transfected with p33ING1 were measured in vitro.  The results were as follows: p33ING1 mRNA expression level in tumor cells was enhanced not only by the 5-FU concentration but also by lapse of time.  The MTT results also proved that high expression of p33ING1 mRNA can increase cell's sensitivity to chemical drug 5-FU.  The detection method based on MB can be used to provide useful evidence quickly and quantitatively for gene expression and new chemical drugs development.

Key words: KeywordsMolecalar beacon;  p33ING1 mRNA; 5-FU; Nasopharyngeal carcinoma; Drug sensitivity

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