CJCU

Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (2): 263.

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Analysis of E6 Variants of Human Papillomavirus Type 18, 45 Isolated from Cervical Cancer Patients in Sichuan Province

WU En-Qi1, REN Yuan2, QIU Ai-Dong1, ZHANG Guo-Nan2, JIANG Chun-Lai1, FAN Ying2, YU Xiang-Hui1, WU Yong-Ge1, ZHA Xiao2, KONG Wei1   

  1. 1. Research Center of Vaccine, College of Life Science, Jilin University, Changchun 130023, China;
    2. Sichuan Cancer Hospital, Chengdu 610041, China
  • Received:2005-03-09 Online:2006-02-10 Published:2006-02-10
  • Contact: KONG Wei. Email: weikong@mail.jlu.edu.cn; ZHA Xiao. E-mail: zha530909@sina.

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Abstract:

The E6 genes of human papillomavirus(HPV) 18 and 45 variants were isolated and amplified by PCR from the tumor tissues of patients with cervical cancer in Sichuan Province of China, and the E6 genes were sequenced for investigating their structural characteristics and variability. The same two point silence mutations were found in three samples of HPV18, and six point mutations were found in one sample of HPV45, resulting in changes of the translated amino acids at two points(T19I and C53F) which are located in the HPV E6 epitope regions. Some point mutations of the HPV45 are identical to those nucleotides found specifically in the HPV18 from these samples, the diversity between HPV18 and HPV45 of Sichuan variants is less by 4.05% than between HPV18 and HPV45 of wild types, this value is lower than that of variants isolated from Africa, which support the hypothesis that the phylogenetic root of HPV18 and 45 is in Africa.

Key words: sHuman papillomavirus(HPV)18; Human papillomavirus(HPV)45; E6 mutation; Cervical cancer

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