Chem. J. Chinese Universities ›› 2006, Vol. 27 ›› Issue (2): 218.
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TIAN Hong-Zhe, Yang Bing-Cheng, Guan Ya-Feng
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Abstract:
Abstract A method was developed for the preparation of static coating onto the fused-silica columns with cross-linked polyacrylamide. The coated columns were effective to suppress the electroosmotic flow and prevent the absorption of basic proteins on the column wall. The basic proteins were separated by using the coated columns at pH=4.0, with retention time variation of less than 2.1% RSD. The average separation efficiencies were 2.4×105 plates/m for alkaloids. The cross-linked polyacrylamide coated columns can be prepared reproducibly by using this method.
Key words: KeywordsCross-liked polyacrylamide-coated column; Capillary electrophoresis; Protein separation; Alkaloid separation
CLC Number:
0658
TrendMD:
TIAN Hong-Zhe, Yang Bing-Cheng, Guan Ya-Feng. Preparation and Application of Cross-linked Polyacrylamide Coated Column for Capillary Electrophoresis[J]. Chem. J. Chinese Universities, 2006, 27(2): 218.
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http://www.cjcu.jlu.edu.cn/EN/Y2006/V27/I2/218
