Abstract: We employed 2-DE with matrix-assisted laser desorption/ionization mass spectrometry(MALDI-TOF-MS) analysis after in-gel digestion protocol to identify the expressing proteins of human squamous lung carcinomas(SQCLC) and small cell lung carcinomas(SCLC) from surgical resection of 16 lung tumor patients. From a total of 59 spots excised from the 2-DE gel, 53 proteins had matches returned from the database search leading to the identification of these proteins. Among them only about 10 proteins have a significant difference(7 for SQCLC and 3 for SCLC, respectively) between SQCLC and SCLC. Meanwhile, 34 proteins, which highly existed in both carcinomas with different expression levels, as well as 6 proteins with almost the same expression levels, were found as the common proteins for both carcinomas. Among these identified proteins, 24 proteins had been previously proposed as the associated proteins with lung carcinomas, and 4 proteins had been linked to the other human carcinomas but not proposed in lung carcinomas.

Key words: Squamous cell lung carcinomas, Small cell lung carcinomas, Proteomics