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Chem. J. Chinese Universities ›› 2005, Vol. 26 ›› Issue (6): 1166.

• Articles • Previous Articles     Next Articles

Monitoring Adsorption in situ and Kinetic Studies of Low-density Lipoprotein onto Cholesterol Modified Dextran Using Quartz Crystal Microbalance

HAN Song-Yan1, LIU Dong-Bo1, HUANG Liang1, WANG Shen-Qi2, LIU Qing-Ping2, Anzai Jun-ichi3, Tetsuo Osa3, CHEN Qiang1   

  1. 1. Life Sciences College;
    2. The Key Laboratory of Bioactive Materials of Ministry of Education, Nankai University, Tianjin 300071, China;
    3. Graduate School of Pharmaceutical Sciences, Tohoku University, Seidai 9808578, Japan
  • Received:2004-06-15 Online:2005-06-10 Published:2005-06-10

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Abstract: The use of quartz crystal microbalance(QCM) for monitoring in situ the adsorption of low-density lipoprotein(LDL) onto cholesterol modified dextran(CMD) was described. The kinetic studies were made. The experimental results show that kinetic curves are fitted for that of Langmuir adsorption equation quite well, when LDL mass concentration(ng/μL) are 9.9, 12.38, 14.14, respectively, and the rate constants for adsorption(mol·L-1·s-1) and adsorption capacity(ng/cm2) are estimated to be (0.047 7), 0.053 6, 0.062 8 and 107.6, 139.6, 167.9, respectively. The maximal LDL adsorption capacity attains 284.8 ng/cm2 and the adsorptive efficiency was calculated as 72.91%.

Key words: Quartz crystal microbalance, Cholesterol modified dextran, Low-density lipoprotein, (Adsorption)

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