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Chem. J. Chinese Universities ›› 2005, Vol. 26 ›› Issue (6): 1046.

• Articles • Previous Articles     Next Articles

Identification of Soluble Resistance-related Calcium Binding Protein by Bio-mass Spectrometric Analysis

LIU Ning1,2, QI Jing3, LV Lei1, LIU Zhi-Qiang1, LIU Shu-Ying1, YANG Chun-Zheng2   

  1. 1. Laboratory of New Drug Research and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
    2. Institute of Life Science and Technology, Jilin University, Changchun 130021, China;
    3. State Key Laboratory of Experimental Hematology, Institute of Hematology, Chinese Academy of Medical Sciences, Tianjin 300020, China
  • Received:2005-01-06 Online:2005-06-10 Published:2005-06-10

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Abstract: The protein profiles of the drug-resistant cell line K562/A02 and its sensitive parental cell line K562 were compared by using high resolution two-dimensional gel electrophoresis with IPG strip of pH 4.7-59. One protein spot was found to be differentially expressed, which was identified as the soluble resistance-related calcium binding protein(sorcin). Tandem mass spectrometric analysis of one tryptic peptide by a TOF-TOF analyzer as well as the western blot analysis substantiates the result derived from peptide mapping fingerprint data.

Key words: Soluble resistance-related calcium binding protein, Matrix-assisted laser desorption ionization time of flight mass spectrometry, Two-dimensional gel electrophoresis

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