Mass Spectrometry Analysis of Recombinant Endostatin and Effect on Cell Cycle of Endothelial Cells

Abstract

Abstract: The structure of recombinant endostatin and mechanism of acting on endothelial cell were studied by mass spectrometry, circular dichroism spectroscopy, fluorescence spectroscopy and cell cycle analysis technique. It was found that the recombinant protein prepared from inclusion body of E. coli may combinate the derivated protein with N-terminal Met. The mechanism by which recombinant endostatin causes G₂ arrest in endothelial cells was observed, which demonstrated that the apoptosis of endothelial cell induced by endostatin was correlated with cell cycle. The results lay the foundation for the antiangiogenesis therapy of the tumor.

Key words: Recombinant endostatin, Mass spectrometry, Circular dichroism spectroscopy, Cell cycle

CLC Number:

O657

TrendMD:

WANG Qun, NI Jia-Zuan. Mass Spectrometry Analysis of Recombinant Endostatin and Effect on Cell Cycle of Endothelial Cells[J]. Chem. J. Chinese Universities, 2005, 26(5): 870.

[1]	ZHU Kai, LI Jie, WU Xiaoyi, HU Weiwei, WU Dongmei, YU Chengxiao, GE Zhiwei, YE Xingqian, CHEN Shiguo. Combined PGC-Triple-Tof-MS Enables the Separation， Identification of Sugar Beet Pectin Derived Oligomers [J]. Chem. J. Chinese Universities, 2022, 43(6): 20220023.
[2]	LIANG Quntao, ZOU Qiang, LIN Jianghui, LIU Shutao, WEI Zheng. Ultraviolet Absorbance of Saturated Structure in the Non-reducing End of Heparin/Heparan Sulfate Disaccharides and Its Application in Sequence of Heparin Oligosaccharides [J]. Chem. J. Chinese Universities, 2021, 42(6): 1776.
[3]	DOU Shuzhen, WANG Zhongshun, LYU Nan. Improving the Detection Performance of Surface-assisted Laser Desorption/ionization Mass Spectrometry by Silicon Nanostructures [J]. Chem. J. Chinese Universities, 2021, 42(4): 1156.
[4]	ZHENG Haijiao, JIANG Liyan, JIA Qiong. Arginine Functionalized Magnetic Nanoparticles and Its Application in Phosphopeptides Enrichment [J]. Chem. J. Chinese Universities, 2021, 42(3): 717.
[5]	ZHAO Huanhuan, LI Cunyu, JIN Hong. Evaluation of Quantitative Phosphoproteomic Performance of Sequential Enrichment of Metal Oxide Affinity Chromatography for Phosphopeptides Using TMT 10-plex Isobaric Labeling [J]. Chem. J. Chinese Universities, 2021, 42(12): 3624.
[6]	WANG Ruxin, ZHAO Zhongjun, HE Feiyao, YUE Hanlu, DENG Fulong, LI Hong, LI Wenwen, DUAN Yixiang. Characteristic Analysis of C₁—C₃n-Aldehydes and n-Alcohols in Proton Transfer Reaction Time-of-flight Mass Spectrometry [J]. Chem. J. Chinese Universities, 2021, 42(12): 3632.
[7]	WANG Baichun, YUAN Yuxin, YAN Yinghua, DING Chuanfan, TANG Keqi. Glucose-6-phosphate Functionalized Hydrophilic Magnetic Probe： a Dual-purpose Affinity Material for Effective Separation and Enrichment of Glycopeptides/Phosphopeptides [J]. Chem. J. Chinese Universities, 2021, 42(10): 3062.
[8]	DENG Jiewei, YANG Yunyun, LIN Li, LUAN Tiangang. Rapid Classification of Daphniamagna and Daphnia pulex by Surface-coated Probe Nanoelectrospray Ionization Mass Spectrometry Lipidomics [J]. Chem. J. Chinese Universities, 2020, 41(9): 2011.
[9]	LI Xiaoqian, ZHANG Hua, LU Haijian, LIU Chang, LIU Qinglong, MA Xiayu, FANG Yuanping, LIANG Dapeng. Mechanism of Photocatalytic Degradation of Rhodamine B by TiO₂ Nanowire Array with Internal Extraction Electrospray Ionization Mass Spectrometry [J]. Chem. J. Chinese Universities, 2020, 41(9): 2003.
[10]	WANG Xiaoyu, YAN Guoquan, ZHOU Xinwen, YANG Pengyuan. Assignment of Polypeptide Disulfide Bonds by Chemical Cleavage and Bio-mass Spectrometry^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1505.
[11]	HE Yu, XIA Qian, WANG Wenli, LUO Fang, CHEN Jinfeng, GUO Yating, WANG Jian, LIN Zhenyu, CHEN Guonan. Triglycerides as Potential Biomarkers for Pesticides Pollution in Aquatic Environment Using UPLC-QTOF-MS ^† [J]. Chem. J. Chinese Universities, 2020, 41(3): 431.
[12]	WANG Tianqi,YU Qiongwei,FENG Yuqi. Analysis of Imidazole Propionic Acid in Serum of Patients with Type 2 Diabetes Based on NiO@SiO₂ Solid-phase Extraction Coupled with Liquid Chromatography-Mass Spectrometry ^† [J]. Chem. J. Chinese Universities, 2020, 41(2): 262.
[13]	AI Wanpeng, SONG Shiyao, BAI Yu, LIU Huwei. Development and Applications of Mass Spectrometry in Reaction Monitoring [J]. Chem. J. Chinese Universities, 2020, 41(12): 2598.
[14]	WANG Xiaoqun, HUANG Guangming. Investigation of Lipid Distributions by Trifluoroacetic Acid-enhanced Desorption Electrospray Ionization Mass Spectrometry Imaging [J]. Chem. J. Chinese Universities, 2020, 41(12): 2673.
[15]	WANG Lianping,LI Qingjie,LIU Xiaoyan,REN Yueying,YANG Xiuwei. Screening of Cholinesterase Inhibitors in Fructus Evodiae Alkaloids Based on UFLC-MS/molecular Simulation ^† [J]. Chem. J. Chinese Universities, 2020, 41(1): 111.

Mass Spectrometry Analysis of Recombinant Endostatin and Effect on Cell Cycle of Endothelial Cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments