CJCU

Chem. J. Chinese Universities ›› 2005, Vol. 26 ›› Issue (12): 2259.

• Articles • Previous Articles     Next Articles

Purification and Analysis of Antigencity of Chimeric Antigen of HIV-1

JIANG Jing1, ZHAO Li-Hui1, 2, YU Xiang-Hui1, JIANG Chun-Lai1, WU Yong-Ge1, MAO Pan-Yong3, LI Wei1, KONG Wei1*   

  1. 1. College of Life Science, JiLin University, Changchun 130012, China; 2. College of Agriculture, Jilin Agriculture
    University, Changchun 130118, China; 3. Department of Virology, 302 Hospital of PLA, Beijing 100039, China
  • Received:2005-03-25 Online:1905-03-14 Published:1905-03-14
  • Contact: KONG Wei;E-mail：weikong@jlu.edu.cn

Abstract:

In order to get the high-efficient diagnosing reagent of HIV, we choose three segments of HIV-1 envelope protein as the immune antigen, 469-511aa, 538-674aa and 700-734aa, which include more epitope. These three segment genes were obtained by PCR amplification and cloned into prokaryotic expressing vector. The results show that chimeric env protein can be expressed in E. coli BL21 strain high efficiently. We purified the protein by metal Ni-sepharose 4B, the data of ELISA demonstrate that purified chimeric antigen has good antigenic specificity.

Key words: env Gene; Chimeric antigen; Antigenicity; Immunology diagnosing, env Gene, Chimeric antigen, Antigenicity, Immunology diagnosing

CLC Number: 

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