Abstract: An effective coupling of microdialysis and capillary electrophoresis with laser induced fluorescence detection technique(CZE-LIF) was explored for the analysis of amino acid neurotransmitters in striatum of rat after transient focal cerebral ischemia in group treated with saline and group treated with hydrochloric acid(ligustrazine).The separation of amino acids was achieved within 30 min on an uncoated fused silica capillary(67 cm×50 μm i.d.) column with botate buffer(10 mmol/L,pH=10.35) and an applied voltage of(12.5 kV).With the current method,the fluorescence intensity has linear relationship with each amino acid concentration between 1×10^-6-8-9

Key words: Laser-induced fluorescence, Microdialysis, Amino acid neurotramsmitter, Cerebral ischemia, Striatum