Abstract: An improved system of Hadamard transform(HT) microscopic fluorescence imaging was presented. Based on the measurements of single-cell samples, the resolving ability of the system and the process of image recovery were discussed respectively. The results show that the system can be used for both single-cell morphological and quantitative analysis. Pixel resolution of the HT images is identical in x and y two-dimension, and reaches to the resolving power of the microscope under the same imaging lens, hence the system's imaging ability is reliable when the two-dimensional HT image of micron-scale single-cell samples should be obtained, and the system can be effectively applied to the single-cell morphological analysis. The quantitative measurement of fluorescence bleaching of pollen cells demonstrates that the process of image recovery for all these samples should be initialized, when the quantitative data of a same series of samples provided by different HT images should be mathematically compared.

Key words: Hadamard transform, Spectral imaging, Fluorescence microscopy, Image recovery, Single-cell analysis