Studies on the Interaction Between Catecholamine Derivatives and DNA by Means of Spectroscopic and Voltammetric Methods

Chem. J. Chinese Universities ›› 2004, Vol. 25 ›› Issue (7): 1235.

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Studies on the Interaction Between Catecholamine Derivatives and DNA by Means of Spectroscopic and Voltammetric Methods

YANG Gong-Jun, XU Jing-Juan, CHEN Hong-Yuan

Institute of Analytical Science, State Key Laboratory of Coordination Chemistry, Department of Chemistry, Nanjing University, Nanjing 210093, China

Received:2003-05-21 Online:2004-07-24 Published:2004-07-24

Abstract

Abstract: The interactions between catecholamine derivatives and DNA were investigated by means of cyclic voltammetry, UV-Vis absorption spectra and fluorescence spectra. The results show that the interaction mode is mainly electrostatic interaction at the low concentration of dobutamine and adrenaline whereas intercalative binding plays a dominant role at their high concentration. As for dopamine, it intercalates into the double helix of DNA in the concentration range of 5.00×10^-5 to 9.00×10^-4 mol/L. The binding constants of dopamine, adrenaline and dobuamine with DNA are determined as 1.55×10³, 9.77×10³ and 1.74×10⁴ L/mol, respectively, by electrochemical method.

Key words: ctDNA, Ethidium bromide, Catecholamine derivatives, Spectroscopic and voltammetric methods

CLC Number:

O612.2

TrendMD:

YANG Gong-Jun, XU Jing-Juan, CHEN Hong-Yuan . Studies on the Interaction Between Catecholamine Derivatives and DNA by Means of Spectroscopic and Voltammetric Methods[J]. Chem. J. Chinese Universities, 2004, 25(7): 1235.

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Studies on the Interaction Between Catecholamine Derivatives and DNA by Means of Spectroscopic and Voltammetric Methods

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