Abstract: An improved method of mass compatible dynamic coating was developed for proteins identification with capillary electrophoresis-mass spectrometry(CE/MS), which kept the high separation efficiency of dynamic coating and eliminated the negative effects of coating reagent to MS detection. The identification efficiency of protein by the peptide mapping of tryptic digested protein was improved. The complex protein mixture was analyzed and successfully identified by this method. The identification efficiency of protein by the peptide mapping to tryptic digested protein was dramatically improved, that the MAscot score of myoglobin digest analysis on CE/MS was increased from³04 to 914 with the dynamic coating. Four protein tryptic digests mixture was also analyzed and successfully identified by this method.

Key words: Hydropropyl cellulose, Dynamic coating, Capillary electrophoresis-mass spectrometry(CE-MS), Protein identification