Determination of E3 in Serum by EIA with Biotin-Avidin Amplification System

Abstract

Abstract: The complex of Estriol and Biotin(E3-Biotin) was prepared by a simple procedure. E3-Biotin and horseradish peroxidase-labelled avidin(HRP-Avidin) were regarded as a probe in enzyme immunoassay(EIA). A sensitive EIA was developed and applied to determining E3 in serum. The unspecific adsorption of HRP-Avidin was inhibited by adding the calf serum into enzyme buffer. The experimental results indicated that the higher sensitivity and better stability and repeatability were achieved without any influence from the unspecific adsorption. The calibration curve covered a range from 0.1 to 10 ng/mL,and the limit of detection was 0.06 ng/mL(n=10,3σ/N).

Key words: Estriol, Enzyme immuno assay(EIA), Biotin, Avidin

CLC Number:

O657

TrendMD:

ZHAO Jin-Fu, WANG Yong-Cheng, MI Jian-Qiu, CHANG Wen-Bao. Determination of E3 in Serum by EIA with Biotin-Avidin Amplification System[J]. Chem. J. Chinese Universities, 2004, 25(6): 1019.

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Determination of E3 in Serum by EIA with Biotin-Avidin Amplification System

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