Optimization Method of Multi-segment Gradient for Separation of Ginsenosides in Reversed Phase-High Performance Liquid Chromatography

Abstract

Abstract: A multi-segment gradient method for the separation of ginsenosides in reversed phase-high performance liquid chromatography(RP-HPLC) was developed. The retention parameters of 8 ginsenosides(Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2 and Rd) were obtained on an ODS2 C18 column(4.6 mm×250 mm) from a series of isocratic acetonitrile-water elution. It is found that two-parameter retention equation of ginsenosides can′t be used to accurately predict the resolution, but the three-parameter retention equation can. The reason is that the retention factor k′ decreases fast while the volume fractions of acetonitrile increase in mobile phase, which make the precision of two-parameter retention equation can′t satisfy the predicting need of resolution. Based on the three-parameter retention equation, an optimum elution program consisting of three segments was developed. Under the optimized gradient conditions, the retention time and resolution of ginsenosides are in good agreement with the predicted ones. The analysis time of ginsenosides is shortened and the resolution is improved by the method.

Key words: High performance liquid chromatography(HPLC), Retention equation, Gradient optimization, Ginsenoside

CLC Number:

O658

TrendMD:

MA Xiao-Qiong, WANG Long-Xing, XU Qing, ZHANG Feng, XIAO Hong-Bin, LIANG Xin-Miao . Optimization Method of Multi-segment Gradient for Separation of Ginsenosides in Reversed Phase-High Performance Liquid Chromatography[J]. Chem. J. Chinese Universities, 2004, 25(2): 238.

References

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