Studies on GC/MS and ESI/MS/MS Screening of MMAUria with Stable Isotope Labeling Method

Abstract

Abstract: Early diagnosis of inborn errors of metabolism(IEM) is a challenging and undeveloped area in China.As one of the diseases with most frequency, methylmalonic aciduria(MMAUria) is studied by using GC/MSand ESI/MS/MSmethod.Information of almost all urinary metabolites can be obtained by GC/MSusing urease pretreatment and stable isotpe labeling.We also develop a way to analyze a urine sample in 2 minutes by ESI/MS/MS.Both the two methods have a good recovery.The combination of the two methods can not only be used for high-risk screening, but also for newborn screening.

Key words: GC/MS, ESI/MS/MS, MMAUria, Methylmalonic acid, Methylcitric acid

CLC Number:

O657

TrendMD:

ZHAO Ji-Yuan, WANG Yi-Ming, LUO Guo-An, ZHANG Ting, WU Jian-Xin, WANG Li-Wen . Studies on GC/MS and ESI/MS/MS Screening of MMAUria with Stable Isotope Labeling Method[J]. Chem. J. Chinese Universities, 2003, 24(8): 1368.

[1]	SUN Ping, LIANG Qiong-Lin, WANG Yi-Ming, LUO Guo-An ZHANG Ting, WANG Li-Wen . Rapid Detection of Methylmalonic Acidemia by Derivation and Electrospray Tandem Mass Spectrometry [J]. Chem. J. Chinese Universities, 2005, 26(3): 421.
[2]	GONG Fan, LIANG Yi-Zeng, SONG You-Qun, PENG Yuan-Gui, Leung-Alexander-Kaiman, Chau-Footim . Determination of Volatile Components of Ping-wei Powder(Ⅱ) --Analysis of the Volatile Oil in Cortex Magnoliae Officinalis with GC/MS [J]. Chem. J. Chinese Universities, 2001, 22(9): 1481.
[3]	MA Ting, LI Gong-Ke, LI Xiao-Dong, TIAN Xue-Mei, ZHANG Zhan-Xia, BAO Lun-Jun, ZHOU Hui-Tang. Quantitative Determination of Resveratrols in Wine by GC/MS and Elementary Study on Their Physiological Activity [J]. Chem. J. Chinese Universities, 2000, 21(7): 1023.
[4]	XU You-Xuan, SHEN Li, WANG Shan, WU Yun, ZHANG Chang-Jiu . Studies on the Analysis of β-blockers and Their Metabolites in Urine by Gas Chromatography (Ⅲ)──Analysis of Five β-Blockers and Their Metabolites in Rat'Urine by Solid-phase Extraction and GC/MS [J]. Chem. J. Chinese Universities, 1995, 16(3): 384.
[5]	XU You-Xuan, ZHANG Chang-Jiu, SHEN Li, WU Yun, WAN Shan. A Study of the Conformation of Some β-blockers [J]. Chem. J. Chinese Universities, 1995, 16(1): 75.
[6]	YAN Ji-Chang, ZHANG Hong, XU Shao-Min, WEI Yong-Di, YAN Xue. Studies on the Volatile Oil Composition of Rhodiola Using GC/Ms/DS and GC/MS/DS [J]. Chem. J. Chinese Universities, 1994, 15(8): 1175.
[7]	MA Yu-Guang, GUO Hai-Chen, YANG Mei-Lin, SHEN Jia-Cong. Determination of the Initiating Active Species of Plasma Initiated Polymerization by Gas Chromatograph/Mass Spectrometry [J]. Chem. J. Chinese Universities, 1993, 14(7): 1019.
[8]	GUI Kai-Rong, ZHOU Yi, ZHANG Chang-Jiu, CHEN Shi-Zhi. Determination of 4 Drugs of Ephedrines in Urine by GC/MSD [J]. Chem. J. Chinese Universities, 1992, 13(12): 1553.
[9]	Ma Xi-zhong, Yu Xiao-bing, Zheng Zhen-hua. Mao Jin-song. Determination of Components from Herba Cistanches by Analytical Supercritical Fluid Extraction with GC/MS [J]. Chem. J. Chinese Universities, 1991, 12(11): 1443.
[10]	Chen Yiaozu, Li Zhaolin, Wang Mingkui, Chen Ning, Qiu Ningying. A Study on the Chemical Constituents of Volatile Oils by Capillary GC/MS and GC/FTIR [J]. Chem. J. Chinese Universities, 1990, 11(9): 963.
[11]	Li Zhaolin, Wang Mingkui, Chen Ning, Li Yuanzhong, Chen Yaozu . Study on Chemical Constituents of Volatile Oils of Three Species of Rabdosias from Tibet [J]. Chem. J. Chinese Universities, 1990, 11(2): 208.

Studies on GC/MS and ESI/MS/MS Screening of MMAUria with Stable Isotope Labeling Method

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 11

Recommended Articles

Metrics

Comments