Influence of Rare Earth Irons on the Molecular Recognition Between Calmodulin and Monoclonal Antibody

Abstract

Abstract: The molecular recognitions between monoclonal antibody 2C3 and calmodulin saturated with different metaLIons(La³⁺, Eu³⁺, Yb³⁺and Ca²⁺) were investigated by the enzyme linked immunosorbent assay(ELISA) and labeled fluorescence spectroscopy.The results indicate that after binding with different metaLIons, the calmodulin undergoes different conformational changes, which have a significant effect on its recognition ability to a metaLIon induced conformation-specific monoclonal antibody.The dissociation constants between the antibody and the calmodulin saturated with La³⁺, Eu³⁺, Yb³⁺are (26.8±2.5), (21.8±3.4) and (64.8±5.1) nmol/L, respectively, while the dissociation constants between the antibody and Ca²⁺-CaMor apoCaMare (157±4.2) and (177.2±2.8) nmol/L, respectively.This result indicates lanthanide-induced conformational changes of calmodulin are not similar with that induced by calcium, which maybe account for the facts that lanthanide and calcium ions lead to different effects on the regulating function of calmodulin in biological systems.

Key words: Rare earth ion, Calmodulin protein, Monoclonal antibody, Molecular recognition

CLC Number:

O657.3

TrendMD:

LI Wei-Guo, QI Chao, DU Li, LIU Li-Yan, ZHAO Da-Qing. Influence of Rare Earth Irons on the Molecular Recognition Between Calmodulin and Monoclonal Antibody[J]. Chem. J. Chinese Universities, 2003, 24(8): 1346.

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Influence of Rare Earth Irons on the Molecular Recognition Between Calmodulin and Monoclonal Antibody

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