CJCU

Chem. J. Chinese Universities ›› 2003, Vol. 24 ›› Issue (7): 1198.

• Preface • Previous Articles     Next Articles

Enzyme-linked Fluorescence Immunoassay for Human IgG by Using a pH-Sensitive Phase Separating Polymer as Carrier

LIN Peng1,2, ZHENG Hong1, YANG Huang-Hao1, YANG Wei1, ZHANG Chang-Gong3, XU Jin-Gou1   

  1. 1. Key Laboratory of Analytical Science of MOE, Department of Chemistry, Xiamen University, Xiamen 361005, China;
    2. Aquaculture College, Jimei University, Xiamen 361021, China;
    3. Cancer Research Center, Xiamen University, Xiamen 361005, China
  • Received:2003-04-01 Online:2003-07-24 Published:2003-07-24

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Abstract: Poly(N-isopropylacrylamide-N,N-dimethyl aminopropyl methacrylamide) [P(NIP-DMAPM)], a novel linear water-soluble pH-sensitive polymer whose phase transition pH is about 7.4 at 37 ℃, was synthesized. P(NIP-DMAPM) polymer precipitated out of water above a critical pH 7.4 at 37 ℃ and redissolved at pH below 7.4. P(NIP-DMAPM) was used as a novel separation carrier for the reactants in immunoassay and a enzyme-linked fluorescence immunoassay for human IgG was built. The linear range of determination was 0-1700 ng/mL and the limit of detection was 2.2 ng/mL. The sensitivity of this method was close to that of traditional ELISA by using same reactants. However, the assay was much faster. This method has been applied to the determination of the human IgG levels in human serum with satisfactory results.

Key words: pH-Sensitive phase separating polymer, Enzyme-linked fluorescence immunoassay, Human IgG

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