Abstract: A novel pyrrolidino-porphyrin, meso-5,10,15,20-tetra[4-(N-pyrrolidinyl)phenyl] porphyrin(TBPPH₂), was synthesized based on the porphyrin′s special affinity for cancer cells and the antitumor activity of pyrrolidine compounds. Its structure was characterized by UV-Vis spectra, elemental analysis and ¹HNMR. In order to study the possible anticancer activity of TBPPH₂, the interaction model based on TBPPH₂ and bovine serum albumin(BSA) was built, and the binding mechanism was studied with UV-Vis spectra and fluorescence spectra. The research results indicate that BSA strongly bind to TBPPH₂ with the molar ratio of 1∶1, the hydrophobic force was the main binding force, the shortest binding distance r was 2.4 nm, the equilibrium constant K_A was 2.51×10⁴ L/mol, the reaction entropy ΔS was 84.18 J/(mol·K), the binding constant K_B was 5.13×10⁵ L/mol. The research results show that it is possible for the porphyrins with pyrrolidinyl groups to become a new type of the antitumor drugs.

Key words: meso-5,10,15,20-Tetra[4-(N-pyrrolidinyl)phenyl] porphyrin, Bovine serum albumin, Synthesis, Fluorescence spectra, UV-Vis spectra