A Novel NO Microsensor and Its Application in the Detection of NO in Myocardial Cells

Abstract

Abstract: A novel sensitive, selective and stable nitric oxide(NO) microsensor is described, which is modified by nano Au colloid and Nafion. Determined by Atomic Forced Microscope, the diameter of Au colloid particles is from 7 to 14 nanometers. The detection of NO is based on the nano Au particles catalysis of NO oxidation at an anodic potential of +0.74 V(vs. SCE). The microsensor showed a low detection limit, high selectivity and sensitivity for NO determination. The oxidation current(measured by differential pulse amperometric technique) was linear with NO concentration ranging from 1.0×10^-7 to 4.0×10^-5 mol/L with a calculated detection limit of 5.0×10^-8 mol/L(S/N=3). Using the microsensor, the direct, real time production of NO in the myocardial cells was continuously measured, which showed the NO levels was increased by stimulating with L-arginine and acetylcholine.

Key words: Au colloid, Microsensor, Myocardial cells, Nitric oxide

CLC Number:

O657

TrendMD:

ZHU Min, LIU Min, SHI Guo-Yue, CHEN Jun-Shui, JIN Li-Tong . A Novel NO Microsensor and Its Application in the Detection of NO in Myocardial Cells[J]. Chem. J. Chinese Universities, 2003, 24(2): 245.

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[9]	LEI Lian-Di, ZHANG Hong-Rui, YANG De-Suo, WU Long-Min . Reaction of Hydroxy-substituted Polycyclic Aromatic Hydrocarbons with Nitric Oxide [J]. Chem. J. Chinese Universities, 2004, 25(10): 1863.
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[11]	DING Cai-Rong, Mo Jin-Yuan, CAI Pei-Xiang. A Micro-modified Electrode Detecting Nitric Oxide [J]. Chem. J. Chinese Universities, 1999, 20(9): 1380.
[12]	XIAN Yue-Zhong, YING Xiang-Yang, ZHANG Wen, LUO Min, JIN Li-Tong  . The Research of Nitric Oxide Detection Using Nafion poly N ′, N ″-(1, 3-propanediyliene)bis(1, 2-benzene diamino N, N ′, N ″, N′″- Nickel Microsensor [J]. Chem. J. Chinese Universities, 1998, 19(6): 866.
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[15]	ZHANG Wen-Xiang, JIA Ming-Jun, ZHANG Chun-Lei, WU Tong-Hao, AO Xue-Ju. Selective Catalytic Reduction of Nitric Oxide by Propene over Heteroatom ZSM-5 Zeolites [J]. Chem. J. Chinese Universities, 1996, 17(11): 1773.

A Novel NO Microsensor and Its Application in the Detection of NO in Myocardial Cells

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