Abstract: The uniform zirconia nanoparticle multilayers/micrometer-sized silica particle(ZrO₂/SiO₂) was prepared for HPLC support material by layer-by-layer(LbL)self-assembly technique. XRD pattern shows that the crystalline of nanometer particles on the surface is pure tetragonal zirconia, with a diameter of 13.9 nm. The specific surface area, pore volume and pore size of the material are 172.53m²/g, 0.3784cm³/g, and 8.5 nm, respectively. The novel support not only has the mechanical strength and chemical stability of zirconia, but also has the good chromatographic features such as larger surface area, better permeability, less background pressure.

Key words: Novel HPLC support, Molecular self-assembly, ZrO₂/SiO₂