Abstract: Mitochondrial DNA(MtDNA) was successfully extracted from four individual bones (femurs) from 2 tombs at the ancient Ruins of Jiao he in Turfan basin, dated about 2000～2500 years ago. Through four overlapping primers, we got nucleotide sequence of 363 bp length. Ancient MtDNAwas analyzed by restriction fragment length polymorphism(RFLP) analysis for the A, B, Cand Dhaplogroups and the sequencing of hypervariable region Ⅰ of the MtDNAcontrol region.The result shows that three haplotypes with 7 polymorphic sites in 4 individuals were obtained. Two specimens (male in each tomb) share the same haplotype. It is reasonable to assume that the close blood relationship existed between them. Phylogenetic analysis indicated that Jushi population were scattered among modern Xinjiang Uighuis and did not fall into specific clusters, so our preliminary data imply that Jushi population was not a genetically homogenous population and an ancient mingling of Euro Asian population had existed prior to the early iron age.

Key words: Ancient DNA, Sequence, Restriction fragment length polymorphism analysis, Phylogenetic analysis