Abstract: This paper covers the first application of Dip-Pen Nanolithography(DPN) to directly write protein patterns with submicrometer dimensions onto Au substrate. Using Bovine Serum Albumin(BSA) as the ink in the DPNprocedure, we were able to utilize lateral force microscopy(LFM) images to differentiate between Au substrate and patterned area with deposited monolayers of BSA. Then the first evidence for Au_ Sbonding was reported between the gold substrate and the BSAsurface thiol groups given by the angle resolved XPSmeasurements.

Key words: DPN, Cooperation force, Protein array