Abstract: Apiezoelectric immunosensor has been developed for the detection of Hepatitis Bsurface antigen(HBsAg). Treatment of a Au electrode with cystamine solution results in cysteamine self-assemble monolayers(SAMs). Using a bifunctional cross-linker, glutaraldehyde, the antibody was immobilized on the electrode surface. The influence factors such as the reaction time of immunoreaction and the concentration of antibody etc. were investigated. Under the optimized experimental conditions, a series standard concentration of HBsAg solutions were analyzed in solution. The frequency shift was linear with the antigen mass concentration in the range of 0.2-1.20 μg/mL. The analysis results of two serum specimens showed that the positive serum and the negative serum could be distinguished easily. Glycine HCl solution(0.2 mol/L) was used for desorption of the bound antigen. The crystal could be regenerated nearly 8 times without detectable loss of activity.

Key words: Piezoelectric immunosensor, Hepatieis B surface antigen, Self-assemble monolayers