High Performance Liquid Chromatographic Analysis of Catecholaminein the Microdialysate from Rat Brain

Chem. J. Chinese Universities ›› 2002, Vol. 23 ›› Issue (4): 552.

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High Performance Liquid Chromatographic Analysis of Catecholaminein the Microdialysate from Rat Brain

CHANG Li-Wen¹, XU Gui-Yun¹, HAN Hui-Wan¹, CHANG Xi-Yan², ZHAO Zhuo¹

1. Institute of Chemistry, Center for Molecular Science, Chinese Academy of Sciences, Beijing 100080, China;
2. Beijing Research Institute of Petroleum Processing, Beijing 100083, China

Received:2001-03-21 Online:2002-04-24 Published:2002-04-24

Abstract

Abstract: A method for determination of catecholamine and related metabolite in the microdialysate fromrat brain by HPLC With pulsed amperometric detection was described.The optimum condition of chro-matographic separation and pulsed amperometri detection were studied.Sampling was carried out by mi-crodialysis technique from rat brain.Acomparison of epinephrine(E) content in microdialysates from ratbrain at various event was made.The detection limit of the method is 25 μg/L for epinephrine.

Key words: Catecholamine, Microdialysis, Pulsed amperometric detection

CLC Number:

O657

TrendMD:

CHANG Li-Wen, XU Gui-Yun, HAN Hui-Wan, CHANG Xi-Yan, ZHAO Zhuo. High Performance Liquid Chromatographic Analysis of Catecholaminein the Microdialysate from Rat Brain[J]. Chem. J. Chinese Universities, 2002, 23(4): 552.

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High Performance Liquid Chromatographic Analysis of Catecholaminein the Microdialysate from Rat Brain

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