Preparation and Properties of Abzyme with Thyroxine Deiodinase

Abstract

Abstract: The abzyme (Se-6E8) with a higher thyroxine deiodinase activity was prepared by modifying the serine residues of monoclonal antibody (6E8) with phenylmethanesulfonyl fluoride and sodium hydrogen selenide, and the 6E8 against O-methyl-T₄, which is a kind of thyroxine derivatives and was taken as a hapten for the first time. Two bands were found corresponding to the 5.5 kD heavy chain and the 2.7 kD light chain respectively by SDS-PAGE. The characteristics of dissociation constants, pH, and temperature were also studied. The results show that the activity of Se-6E8 is 2010 U/μmol protein , and the proper temperature and pH of the catalytic reactions is 57 ℃ and 8.2 respectively.

Key words: Selenium, Thyroxine, Deiodinase, Abzyme

CLC Number:

R392.11

TrendMD:

SUN Li-Wei, LIU Li-Yan, ZHAO Da-Qing . Preparation and Properties of Abzyme with Thyroxine Deiodinase[J]. Chem. J. Chinese Universities, 2002, 23(12): 2217.

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Preparation and Properties of Abzyme with Thyroxine Deiodinase

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