Abstract: Several liquid matrixes with different compositions were formed by using some matrice compounds including nanoparticles, and their applications in matrix assisted laser desorption/ionization time of flight mass spectrometry were systematically investigated with several kinds of compounds such as peptide, protein, macrocycle oligosaccharide, small organic molecules as the testing samples. The similarities and differences between solid and liquid method of sample preparation were explored. It is shown from the experimental results that some liquid matrixes possessed a good versatility and the others are especially efficient to the analysis of particular compounds.

Key words: MALDI-TOF-MS, Liquid matrix, Sample preparation