Abstract: p-Hydrazinebenzenesulfonic acid was explored as a novel pre-capillary labeling reagent for CEof mono and disaccharides. The derivatization and the separation in borate buffer were investigated in detail. The derivatization took less than 10 min and the mass detection limit at 200 nm reaches 17.6 fmol of glucose, corresponding to concentration limit of 3.6 μmol/L. The baseline separation of 9 mono and disaccharides could be achieved within 11 min, using a running buffer composed of 100 mmol/Lborate at pH 10.24 This approach is also suitable for quantitation with linear coefficients higher than 0.992 at the concentrations between 0.1-9.1 mmol/L.

Key words: High performance capillary electrophoresis, Carbohydrate, p-Hydrazinebenzenesulfonic acid, Pre-capillary derivatization