Abstract:

The phosphopeptide and corresponding nonphosphopeptide was synthesized with the Fmoc strategy solid-phase synthesis method. Using N,N-diethyl-dialkyl-phosphoramidity as phosphorylating reagent, some phosphopeptides were obtained by the use of the golble phosphorylation approach, others were obtained from the phosphorylated building block. The peptides were purified with HPLC. The longer the phosphopeptide, the lower the phosphorylating efficiency, Up to now, this Fmoc strategy solid-phase synthesis method with the step-wise phosphotylation approach only can be used in the synthesis of phosphopeptide with Tyr.

Key words: O-phosphopeptide, Fmoc strategy solid-phase synthesis, the golble phosphoiyiation approach, the step-wise phosphotylation approach