Studies on High-resolution Hadamard Transform Microscopic Image Detection System Using One-dimensional Mechanical Mask

Chem. J. Chinese Universities ›› 2001, Vol. 22 ›› Issue (7): 1114.

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Studies on High-resolution Hadamard Transform Microscopic Image Detection System Using One-dimensional Mechanical Mask

CHEN Guan-Quan, TANG Hong-Wu, ZHOU Jin-Song, WU Qiong-Shui

Centre of Analysis and Measurements, School of Chemical and Molecular Science, Wuhan University, Wuhan 430072, China

Received:2001-02-08 Online:2001-07-24 Published:2001-07-24

Abstract

Abstract: Anovel Hadamard transform(HT) microscopic image detection system which can generate 511× 512 pixel format images of fluorescence or absorptive light for small samples has been developed, when spatial multiplexing is performed with a 511-slit one-dimensional Hadamard mask and a highly sensitive linear CCD. The weak native fluorescence imaging for Zephyranthes candida (Lindl.) Herb. Pollen cells can be realized within 1 min. The spatial resolution of 06 μm per pixel has been achieved with a 40× objective. The imaging speed, sensitivity and spatial resolution are satisfactory, hence the system has a good application prospect in cell biology and medicine. Some factors that influence imaging speed and quality have been discussed preliminarily.

Key words: Hadamard transform, Image analysis, Fluorescence microscopy

CLC Number:

O657

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CHEN Guan-Quan, TANG Hong-Wu, ZHOU Jin-Song, WU Qiong-Shui . Studies on High-resolution Hadamard Transform Microscopic Image Detection System Using One-dimensional Mechanical Mask[J]. Chem. J. Chinese Universities, 2001, 22(7): 1114.

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Studies on High-resolution Hadamard Transform Microscopic Image Detection System Using One-dimensional Mechanical Mask

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