Abstract: Aprocedure for the preparation of continuous rod consisting of poly(glycidyl methacrylate-co-ethylene dimethacrylate) for immobilized metal affinity chromatography (IMAC) is presented. When chelating Cu(Ⅱ), Zn(Ⅱ) or Ni(Ⅱ) on it, the rod displayed a property of IMACand the selectivity for protein separation was different from that obtained from the naked rod. An abnormal increase in retention times of proteins was found under the condition of either very high pHor very low pHon the metal chelated rods.

Key words: Immobilized metal affinity chromatography, Continuous rod column, Biopolymers