Phase Behavior and Protein Partitioning in Aqueous Two-phase Systems Formed by Mixtures of Polymer and Cationic-anionic Surfactants

Abstract

Abstract: Anovel aqueous two-phase system can be formed by the mixtures of a polymer and cationic-anionic surfactants. Such a system can be used as a partitioning system of proteins. In this work, we investigated the formation, phase behavior and protein partitioning in aqueous two-phase systems formed by dodecyltriethylammonium bromide/sodium dodecylsulfate/PEGand dodecyltriethylammonium bromide/sodium dodecylsulfate/dextran. The ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. It was shown that the surfactants and polymers are enriched in different phases of aqueous two-phase systems. Phase separation are promoted by increasing temperature and adding inorganic salts. Different proteins are partitioned in different phases. The selectivity of protein partitioning is increased by adding ligands with affinity.

Key words: Aqueous two-phases, Protein partitioning, Cationic-anionic surfactants

CLC Number:

O647.1

TrendMD:

XIAO Jin-Xin, BAO Yan-Xia, FANG Lei . Phase Behavior and Protein Partitioning in Aqueous Two-phase Systems Formed by Mixtures of Polymer and Cationic-anionic Surfactants[J]. Chem. J. Chinese Universities, 2001, 22(1): 112.

[1]	LU Hai-Wei, LU Qing-Hua, CHEN Wan-Tao, XU Hong-Jie, YIN Jie . Influence of Nanostructured Polymer Surface on Cell Growth [J]. Chem. J. Chinese Universities, 2003, 24(12): 2324.
[2]	HE Chun-Ying, WU Yi-Qun, SONG Ying-Lin, WANG Yu-Xiao, ZUO Xia . Synthesis and Properties of Tetrabromo-2,3-naphthalocyanine Lead(Ⅱ) [J]. Chem. J. Chinese Universities, 2003, 24(8): 1360.
[3]	XU Shan-Dong, ZENG Qing-Dao, QIAO Ying-Hong, WANG Chen, WAN Li-Jun, BAI Chun-Li . Effect of Length of Substituted Alkyl Chain on 2-D Nanostructure [J]. Chem. J. Chinese Universities, 2003, 24(6): 1085.
[4]	LI Gan-Zuo, CHEN Wen-Jun, GU Qiang, LI Ying, GE Shang-Zheng . Studies on the Dynamic Surface Tension of Dynol-604 [J]. Chem. J. Chinese Universities, 2002, 23(11): 2117.
[5]	CHAI Jin-Ling, XU Jun, LI Gan-Zuo, ZHANG Gao-Yong, LI Ying, CHEN Wen-Jun . Adsorption Equilibrium and Kinetics of Alkyl Polyglucoside on the Air/Water Interface [J]. Chem. J. Chinese Universities, 2002, 23(10): 1915.
[6]	YANG Yan-Lian, ZHANG Jin, NAN Xiao-Lin, LIU Zhong-Fan . Studies on the Dissociation Behavior of Carboxylic Groups at the Open End of Single-Walled Carbon Nanotubes by Tip Chemistry [J]. Chem. J. Chinese Universities, 2002, 23(3): 469.
[7]	HE Chun-Ying, HUO Li-Hua, WU Yi-Qun, ZUO Xia, XI Shi-Quan. Preparation And Gas Sensitivity of Octa-n -butoxy-2,3-naphthalocyanine Copper(Ⅱ) Langmuir-Blodgett Film [J]. Chem. J. Chinese Universities, 2002, 23(1): 14.
[8]	ZHU Zi-Hua, SHENG Xiao-Xia, ZHANG Zhi-Jun, ZHU Tao, LIU Zhong-Fan . Surface Enhanced Raman Spectroscopic Detection of Porphyrin Monolayer [J]. Chem. J. Chinese Universities, 2001, 22(8): 1368.
[9]	LEI Xiao-Jun, CHEN Hai-Feng, LIU Zhong-Fan  . STM Thermochemical Hole Burning Memory——Influence of Pulse Voltage and Duration on Hole Size [J]. Chem. J. Chinese Universities, 2001, 22(7): 1222.
[10]	HAN Guo-Bin, WU Jin-Tian, XU Xiao-Ming, WEI Guang . Studies on the Relation of Black Film Thickness and Film Tension [J]. Chem. J. Chinese Universities, 2001, 22(1): 115.
[11]	FU Qiang, ZHANG Hua, HE Hui-Xin, LIU Zhong-Fan . Force Titration of ω-Mercapto-n-undecylamine Self-assembled Monolayers by Chemical Force Microscopy [J]. Chem. J. Chinese Universities, 2000, 21(11): 1738.
[12]	XU Guo-Hua, Higashitani Ko . Formation of OTS Self-assembled Monolayer on Glass Surface Investigated by AFM [J]. Chem. J. Chinese Universities, 2000, 21(8): 1257.
[13]	ZHANG Liang, MAO Kun-Yuan, ZHANG Xin-Sheng, WANG Jian-Qi . Analysis of the Compositions on PET Surface Layer Modified by Fluorocarbon Plasmas with Angle-dependent XPS Technique [J]. Chem. J. Chinese Universities, 1999, 20(10): 1605.
[14]	ZHANG Hao-Li, GUO Yun, LI Hu-Lin, YANG De-Quan. AFM Controlled Selective Deposition of 1-Octadecanethiol on Gold [J]. Chem. J. Chinese Universities, 1999, 20(9): 1460.
[15]	CHEN Hai-Feng, WANG Jian, CAI Sheng-Min, LIU Zhong-Fan . Self-assembled Au Nanoparticles on GaAs and Its Surface Enhanced Raman Scattering(SERS)-activity [J]. Chem. J. Chinese Universities, 1999, 20(4): 620.

Phase Behavior and Protein Partitioning in Aqueous Two-phase Systems Formed by Mixtures of Polymer and Cationic-anionic Surfactants

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments