Chem. J. Chinese Universities ›› 2000, Vol. 21 ›› Issue (S1): 87.
• Atomic Spectrometry • Previous Articles Next Articles
LIN Xin-Hua, LI Chun-Yan
Online:
Published:
Abstract:
Only trace of quinhydrone, a urease-inhibitor, can inhibit enzymatically promoting hydrolytic reaction of urea[1]. A new enzymatic inhibition kinetic spectrophotometry method[2] for determination of trace quinhydrone was obtained by urea and P-dimethylamino-benzadehyde (color reagent) developing action. In this reaction, maximal absorptive wavelength is 420 nm. The enzymatic promoting reaction rate, log (A0/A1),enzymatic inhibition reaction rate, log (A0/A2,and their difference, log (A2/A1), are measured by detecting the remains of urea. All factors (urease, urea and color reagent dosage; reaction temperature; heating time), effecting log (A2/A1) were investigated.
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LIN Xin-Hua, LI Chun-Yan. Determination of Trace Quinhydrone by Enzymatic Kinetic Spectrophotometry[J]. Chem. J. Chinese Universities, 2000, 21(S1): 87.
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http://www.cjcu.jlu.edu.cn/EN/Y2000/V21/IS1/87