Abstract:

The analysis of hydrolysis mixture derived from heteropolysaccharides is often complicated by the presence of alduronic acids in addition to the aldioses normally resent. Aldoses can easily be determination by gas chromatography (GC) as the alditol acetates^[1], aldononitrile acetates^[2] or trimethylsilyl (TMS)^[3] derivatives. Alduromic acids are most often analyzed spectrophotometrically with carbazole or m-hydroxydiphenyl^[4] etc. These commonly used methods are non-specific in that they do not identify which acid is being analyzed.