Abstract: The separation of fructose from fructose-glucose syrup by using macroporous dihydroxyborylated polystyrene resin(MDPR) has been amplified to get fructose syrup with a high purity. The absorbing and eluting conditions has been optimized, i.e., the ratio of length to diameter of the column was reduced from 175: 1 to 40: 1, which was applied to CSEPchromatography separation system. By applying this system, the purity of the final separated fructose was 95% with a concentration of 35%, and the recovery ratio reached 90%. This kind of resin showed promise in industrial application.

Key words: Macroporous dihydroxyborylated polystyrene resin, Fructose-glucose syrup, Separation of fructose and glucose, Separating degree